![]() ![]() It works on the principle of hybridization. Western blotting is used to find out a specific amino acid or protein sequence. Southern blotting is used to find out a specific DNA sequence. ![]() Western blotting was developed by George Stark’s group at Stanford University in 1979. Southern blotting was developed by Edward M. Comparison Chart Southern BlottingĪ technique that is used to find out a specific DNA sequence in a given mixture is known as southern blotting.Ī technique that is used to find out a specific amino acids sequence of the protein in a given mixture is known as southern blotting. Southern blotting is used to find out a specific DNA sequence whereas, western blotting is used to find out a specific amino acid or protein sequence. This name was used to match with southern blotting. On the other hand, western blotting was developed by George Stark’s group at Stanford University in 1979. Southern blotting is the type of blotting that is used to detect DNA while western blotting is used to find out protein. There are different types of blotting on the basis of detection of specific material, i.e., southern blotting, northern blotting, and western blotting. These molecules are pressed against the surface of an immobilized membrane which transfers the molecules onto the membrane. ![]() Here, minute particles will move faster than the bigger ones. In this technique, macromolecules like nucleic acids (DNA and RNA) and proteins in a mixture move through a slab of gel. Western Blottingīlotting is a technique that is used by scientists to separate different types of molecules from a mixture or sample. The main difference between Southern Blotting and Western Blotting is that Southern Blotting is a technique that is used to detect a specific DNA fragment in a given sample whereas Western Blotting is used to find out a specific protein in a given sample. ![]()
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